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KMID : 0613820020120030242
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Journal of Life Science
2002 Volume.12 No. 3 p.242 ~ p.249
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Optimization of Large Scale Culture Conditions of Bacillus ehimensis YJ-37 Antagonistic to Vegetables Damping-off Fungi
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Joo Gil-Jae
Kim Jin-Ho
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Abstract
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The optimal culture conditions in 500㎖ flask cuture, 5ℓ jar fermenter and 2,000ℓ large scale culture were investigated to maximize the production of antibiotic on Rhizoctonia solani AG4, the causal agent of vegetables damping-off, by the strain Bacillus ehimensis YJ-37. Starch (1.5%) as a carbon source, peptone (0.6%) as a nitrogen source and MgCl₂ (0.15%) as a metal ion in the medium containing Na₂HPO₄ (0.3%) showed the highest production of the antibiotic(s) in a rotary shake (200 rpm). Optimal initial pH of the culture medium, culture temperature and culture time for the antibiotic(s) production were pH 8.0, 32℃ and 54hrs, respectively. Under the optimal conditions in flask culture, cell growth and antifungal activity (clear zone size) were 1.42 x 108 cfu/㎖ (21g-DCW/ℓ) and 13.9 ㎜, respectively. In 5ℓ jar fermenter (medium 3ℓ), optimal air flow, agitation speed and culture time for the antibiotic(s) production were 2 vvm, 200 rpm and 48hrs, respectively. Under the optimal conditions in 5ℓ jar fermenter, cell growth and antifungal activity were 2.06×108 cfu/㎖ (30g-DCW/ℓ) and 13.4 ㎜, respectively. Under the culture conditions of air flow (30 vvm) and agitation speed (200 rpm) at 32℃ for 10 days in 2,000ℓ large scale culture (medium 1,800ℓ, pH 8.0), cell growth and antifungal activity were 0.81×108 cfu/㎖ (12g-DCW/ℓ) and 8.6 ㎜, respectively.
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KEYWORD
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Antagonistic, Bacillus ehimensis, Rhizoctonia solani AG4
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